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IOBC-WPRS Bulletin Vol. 67, 2011


IOBC-WPRS Bulletin Vol. 67, 2011

Working Group "Integrated Protection and Production in Viticulture".
Proceedings of the Meeting at Staufen im Breisgau (Germany), 01 - 04 November, 2009.
Editors: Agnès Calonnec, Carlo Duso, Cesare Gessler, Hanns-Heinz Kassemeyer, Michael Maixner, Denis Thiéry & Tirtza Zahavi.
ISBN 978-92-9067-242-5 [XVIII + 314 pp.]


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Distribution and host plant preferences of Hyalesthes obsoletus,
the vector of bois noir disease, in Switzerland

P. Kehrli, S. Kessler, S. Schaerer, N. Delabays

Abstract: In Europe, the polyphagous planthopper Hyalesthes obsoletus Signoret (Hemiptera,
Cixiidae) is assumed to be the most important vector of the grapevine yellows disease bois noir,
which is caused by phytoplasmas of the stolbur 16Sr-XII-A group. For a better understanding of the
epidemiology of bois noir in Switzerland, distribution and host plant preferences of H. obsoletus
were studied in the field as well as in the laboratory. A national survey revealed that H. obsoletus is
present in vineyards of southern, western and northern Switzerland; however, no specimens were
caught in the east of Switzerland, where evidence for the disease is lacking. Even though field
bindweed (Convolvulus arvensis L.) was much more abundant in vineyards than stinging nettle
(Urtica dioica L.), H. obsoletus adults were captured almost exclusively on the latter. A second,
more targeted field study confirmed H. obsoletus' clear preference for U. dioica. From June to
September 2008, adults of H. obsoletus were primarily captured on U. dioica. However, few
individuals were also caught on C. arvensis, hedge bindweed (Calystegia sepium L.) and four other
dicotyledons. Emergence traps set up above U. dioica and C. arvensis highlighted that significantly
more nymphs emerged from stinging nettle than field bindweed. Besides, a clear association
between the distribution of U. dioica patches and the incidences of bois noir infected vines could be
observed in a commercial vineyard. In conclusion, there is good evidence that H. obsoletus is also
the most important insect vector of bois noir in Switzerland and that the insect prefers to feed and
develop on U. dioica. Stinging nettle therefore plays a major role in the epidemiology of bois noir
in Swiss vineyards.


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Adult monitoring improves control of the flavescence dorée leafhopper
Scaphoideus titanus in Gironde (France) while using less pesticide!

M. van Helden, E. Fulchin, A. Verpy, F. Gil , C. Garcia

Abstract: The flavescence dorée is caused by a phytoplasme spread by the leafhopper Scaphoideus
titanus and is present in the south of France. Leafhopper control is based on up to 3 obligatory
insecticide sprayings (T1; T2; T3) using neurotoxic insecticide compounds over large areas
(Imposed Sprayed Area = ISA). Spraying dates are defined according to the observation of the first
larvae (T0) at T1= T0+4 weeks, T2 = T0+6 weeks (T1 and T2 aim the larvae) and T3 = T0+10
weeks (aiming at adults). Such schedules and area definition are very efficient but lack scientific
basis on insect dynamics and migration and encounter opposition from farmers.
Over the last 5 years progress was made to reduce pesticide use while maintaining sufficient
vector control. A project was launched in the Saint Emilion region after two plots were found
contaminated in 2006. 13000ha of vineyards entered the ISA. Farmers and regional Plant protection
service organised into a small association to test a more ‘integrated’ approach for vector control.
Spraying zones were limited to a buffer zone of 2 km around the contaminated plots. Farmers
between 500m and 2 km were allowed to reduce sprayings to only 1 single application at T0 + 5,
while adult trapping was used to monitor overall results. Traps were yellow delta traps (Piège
Tri-nglué) that are highly efficient. In most of this zone a single insecticide spray reduced trap
catches to almost nonexistent, considered as a proof of sufficient control of the vector. In some
areas trap catches were considered too important (>3 adults on a single trap in a single week) and a
second spraying (T3) was imposed. This was probably due to some farmers either not spraying at
all or organic farmers that only were allowed Rotenone, that is rather inefficient. This approach was
repeated in 2008.
Total cost of trapping and monitoring of larvae (traps, technicians, monitoring) is around 3 to 4
Euros per ha. These costs were largely compensated by the reduction in spraying (64 and 72 percent
reduction in 2007 and 2008 compared to the traditional approach). Moreover, the combination of
reduced spraying and vector monitoring seemed well accepted by the farmers.
In addition all plots were monitored for plants showing symptoms of phytoplasm, around 1200
samples were analyzed first year. Over 80% of the samples appeared to be ‘Bois Noir’ phytoplasm
but some Flavescence Dorée samples were found in both 2007 (2 samples) and 2008 (6 samples).
Scouting and analysis costs were around 25 Euro per ha.
In other areas where spraying frequency was reduced simultaneously but without vector
monitoring, a strong increase in FD symptoms was observed, together with high insect populations.
This probably reveals that many farmers do not apply the single imposed spraying. The trapping
seems a useful tool both to show efficient vector control and to incite farmers to respect the
minimal spraying frequency.


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Effectiveness of hot water treatments against the eggs of Scaphoideus titanus Ball
C. Linder, L. Schaub, F. Klötzli-Estermann

Abstract: The leafhopper Scaphoideus titanus (Hemiptera: Cicadellidae) is the principal vector
of flavescence dorée, a severe grapevine disease spreading in Europe. The project PROPSCAPH
united four European research institutes in order to improve risk management schemes against
the spread of flavescence dorée's vector on propagation material. Amongst others, the impact of
the standard hot water treatment against the disease on the development of the S. titanus eggs was
studied. In autumn 2008, one-year-old and two-year-old cuttings were collected in S. titanus
infested vineyards in France, Italy and Switzerland and sent to our institute. After storage in a
cold chamber until March 2009, half of the cuttings were exposed for 45 minutes to a hot water
treatment at 50°C. Thereafter, treated and untreated cuttings were placed in cages and the
hatching of S. titanus nymphs was assessed. At least 10-times more nymphs hatched from twoyear-
old cuttings than from the one-year- old cuttings. This confirmed the ability of S. titanus to
lay eggs on propagation material. However, hot water treatments significantly reduced the
number of hatched nymphs, e.g. the exposure to hot water killed about 80% of deposited eggs. In
conclusion, hot water treatments do not only have a major impact on flavescence dorée, they also
kill most of its vector's eggs. Thus, the hot water treatment of rootstocks and scions before
grafting is strongly recommended in order to reduce the risk of S. titanus' propagation. Before
commercialisation grafts should be hot-water treated once again, since the chemical protection of
rootlings is not always 100% effective in the nursery fields. All these measures together will
ensure the propagation of plant material free of flavescence dorée and they will minimise the risk
of disease’s and vector's spread.


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Downy, powdery mildew and botrytis risk assessment by climate parameters,
pest and spores monitoring in Rioja Alavesa vineyard (Northwest of Spain)

A.-M. Díez-Navajas, A. Ortiz-Barredo

Abstract: The integrated production Regulation for grapevine in the Basque Country was
published in 2004 (BOPV 221/2004, official bulletin). A previous 3-year study was carried out to
evaluate the real situation of growers to assume this regulation in their exploitations. Several
deficiencies were reported, including technical and management aspects. This work is focused in
Rioja Alavesa, an area with 12,869ha of vineyard in the Northwest of Spain producing
81,325,000 wine grape kilo. Integrated disease control interest is rising, although the number of
fungicides applications in the last two years against downy mildew and other fungal diseases
reached up to 12 treatments, being usual 5 or 6 per season. These data prompted us to implement
the strategy of fungal disease control. We installed 12 monitoring weather stations in different
trials, according to different environment and geographical characteristics. The stations were
equipped with sensors of temperature, relative humidity, leaf wetness, soil temperature, radiation
sensors, rain gauge, and an anemometer. Registered data can be discharged and recuperated from
internet, at the same time that the risk assessment for downy and powdery mildew and botrytis
diseases. In parallel, one sticky glass trap to capture spores by impact was installed near each
station and two more traps in each field. Pheromone traps to capture Lobesia botrana were also
distributed in the fields close to the spore traps, as the abundance of this insect gives an idea of
the possible future botrytis damages and spread. After these assays we would be able to validate
the stations for each studied zone and assure that the risk assessment made by climate parameters
follows a real model, taking into account the disease incidence in the field as well as the spore
and vector populations.


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Biological control of Heliococcus bohemicus Sulc. (Hemiptera: Pseudococcidae)
with the natural enemy Ericydnus sipylus (Walker) (Hymenoptera: Encyrtidae)

G. Sentenac, P. Kuntzmann, L. Perez, A. Gili, P. Kreiter

Abstract: The control of mealybugs by growers currently relies on the use of neurotoxic
insecticides. The possible use of the predator Chrysoperla lucasina (Lacroix) is the unique
alternative method. We propose to evaluate the biological activity in situ of the bred Ericydnus
sipylus (Walker), major parasitoid of Heliococcus bohemicus Sulc. The releases of parasitoids
were carried out in 2007 and 2008.
During the 2007 season, the first three releases of E. sipylus did not appear to have any
effect on the population of mealybugs. However the fourth and fifth release made later in the
season did show significant differences between the experimental plots. The biological control
showed positive results in 2008 and 2009, as the number of H. bohemicus in the “release” blocks
was significantly lower than those of the “control” blocks. A reduction of 50 to 60% of the
number of H. bohemicus was found for the 2007 winter generation and from 75 to 85% for the
2008 summer generation. Despite of stopping the release of E. sipylus rates of 80% were found in
the 2008 winter generation, and 80% in early 2009 summer generation.
If the difference of the population of Pseudococcidae between the experimental plots was
due to the introduction of the parasitoid E. sipylus, the lower level of the population found in
summer 2008 and 2009 in the “control” blocks was probably due to: a) the presence of other
parasitoids like Anagyrus szodensis or predators like Exochomus quadripustulatus (Linné),
Hippodamia variegata Goeze, Harmonia axyridis (Pallas), Coccinella septempunctata Linné,
Nephus quadrimaculatus (Herbst) and Chrysoperla carnea (Stephens) b) the dispersion and
activity of E. sipylus beyond the plots where it was introduced.


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Experiences about the effect of growth regulators on cluster structure
and fruit rot diseases of the vine varieties Pinot Meunier, Pinot Blanc,
Pinot Noir and Rhine-Riesling

K. Bleyer and W. K. Kast

Abstract: In field trials, effects of the growth regulators Gibb 3 and Regalis® on cluster structure
of grapes and the reduction of botrytis disease and sour rot disease were evaluated. Gibb 3 can
only be used on Pinot-varieties. Regalis® also can be used on Rhine-Riesling. The growth
regulators Gibb 3 and Regalis® were very effective in loosening the cluster structure at Pinotvarieties.
Reduction of botrytis disease was compared to fungicide treatments. In most cases, the
yield of grapes decreased without an increase of the sugar content. Results of Regalis® on Rhine-
Riesling varied greatly without relevant reasons. No differences were found when Regalis® was
applied with different amount of water and at different development stages. Gibb3 proved to have
a better effect if applied with greater amounts of water. The quality of red wines increases
because of higher anthocyan- and phenol-contents. White wines produced from grapes treated
with Regalis® didn’t show better tasting results in spite of lower yield.


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Control of grey mould by application of gibberellin acid inhibitor (Regalis®)
and management practices in Vitis vinifera (cv. Riesling)

O. Baus, A. Reineke, and B. Berkelmann-Löhnertz

Abstract: Grapevine varieties with tight clusters are extremely susceptible to infection by grey
mould (Botrytis cinerea). Severe pathogen attack leads to reduced yield and affects wine quality. A
couple of management practices can reduce tightness of berry clusters, among them moderate
defoliation and the use of plant growth regulators. Prohexadione-Calcium (trademark Regalis®) is a
gibberellin acid biosynthesis inhibitor with low toxicity and persistence in the plant. Over a period
of three consecutive years the effect of prohexadione-Ca on grape cluster architecture was tested in
cv. Riesling and combined with anti-Botrytis fungicide applications and leaf removal measures.
While prohexadione-Ca treatments significantly increased the flexibility of grape clusters in 2007,
no significant effects were evident in 2008 and 2009. However, even a minor reduction of index
values of 0.2 – 0.3 may have an influence on the tightness of clusters and thus on the susceptibility
to B. cinerea infections. In all three years severity of B. cinerea was significantly reduced in
Riesling grapes when the following measures were combined: application of Regalis®, fungicide
treatments and moderate defoliation. Therefore, future strategies to control grey mould should aim
at a combination of different management strategies.


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Variety-dependent responses of seeded vine cultivars to different growth regulators
S. Böll, T. Lange, H. Hofmann, P. Schwappach

Abstract: GA-levels and turnover rates during early berry development were much higher and
longer lasting in developing berries of Pinot gris compared to Sylvaner and were negatively
related to their sensitivity to exogenous applied GA3. Anthers and pollen are known to be major
sources of endogenous GAs during prebloom, and as such a higher pollen tube abundance could
be responsible for higher endogenous GA levels during early berry development. Pinot gris and
Sylvaner showed the expected differences in pollen tube abundance that corresponded to the
observed endogenous GA levels. Furthermore, pollen tube analyses of a broad spectrum of
additional cultivars with known GA-sensitivity support the hypothesis, displaying a broad range
of pollen tube abundance that correlates negatively with the known sensitivity of the cultivars to
GA3 applications.
The observed variety-specific pollen tube abundance seems to lead to differential
developmental patterns: postbloom, Pinot gris had no unfertilized ovaries while in Sylvaner up to
35% of the ovaries were unfertilized. Furthermore, there were variety-specific differences in the
degeneration of ovules: Pinot gris had on average 3-4 fertilized ovules per ovary while Sylvaner
only had 1-2 fertilized ovules per ovary. However, during further berry development endosperm
development was aborted in about half of the ovules in Pinot gris, while in Sylvaner all fertilized
ovules developed into seeds. Also in shattered berries the percentage of unfertilized ovaries was
much higher in Sylvaner than in Pinot gris. Altogether, variety-dependent effects were much
stronger than treatment effects.
The results imply that a simple microscopic pollen tube analysis of varieties with hitherto
unknown gibberellin sensitivity might predict the sensitivity to exogenous GA3-treatment
including possible adverse side effects.


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Monitoring nematode populations to adapt fallow periods against
Xiphinema vectors of Grapevine fanleaf virus (GFLV)

M. van Helden, L. Villate, C. Laveau, E. Morin, G. Darrieutort, C. van Leeuwen

Abstract: The nematode Xiphinema index is, economically, the major virus vector in viticulture,
transmitting specifically the Grapevine fanleaf virus (GFLV), the most severe grapevine virus
disease worldwide. A second virus (ArMV) and its vector (X. diversicaudatum) do exist in the
Bordeaux region but seem of lower importance. Plots that have become completely infected, no
longer providing sufficient yield or quality, have to be uprooted to be replaced by new healthy
Increased knowledge of the spatial distribution of this nematode, both horizontally and
vertically, and of correlative GFLV plant infections, is essential for efficient control of the
disease. Vertical distribution data showed that the highest numbers of individuals occurred at 40
to 110cm depth, corresponding to the layers where the highest densities of fine roots were
observed. Horizontal distribution revealed a significant aggregative pattern with patches of 6 to
8m diameter, together with a significant neighbourhood structure of nematode densities, thus
identifying the relevant sampling scale to describe the nematode distribution. Nematode patches
often, but not always, correlate significantly with those of GFLV-infected grapevine plants. The
nematodes are able to survive in the soil even without food for several years. Since nematodes
live deep in the soil, they cannot be attained by nematicides (that are, anyway, progressively
forbidden). Because of this lack of efficient control measures of the vector, a fallow period of 7
years is advised to farmers but rarely observed for economic reasons. In some cases, high reinfestation
levels have occurred with short fallow periods but this does not always occur.
Sampling of numerous plots in the Bordeaux region, uprooted because of virus infestations,
showed that nematode numbers are unpredictable. No clear relation was found between the
percentage of each virus and the population of its vector. X. diversicaudatum was rarely present
in significant amounts, even though infestation percentage of plants with ArMV could be high.
X. index was sometimes present in high numbers (up to 300 per litre of soil) in some samples but
mostly numbers were quite low or nematodes even not detected. These results suggest that the
fallow period can be adapted following nematode sampling. In the case of very low nematode
numbers it could be reduced to 1.5 to 3 years whereas longer periods need to be observed when
high numbers are found. Nematode sampling grid should be rather dense, and sampling should
ideally be done twice, just after uprooting and before replanting. The use of ‘nematicidal’
antagonistic plants against X. index is currently being investigated in our lab.


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”VitiMeteo” – a future-oriented forecasting system for viticulture
G. Bleyer, H.-H. Kassemeyer, M. Breuer, R. Krause, O. Viret, P.-H. Dubuis, A.-L. Fabre, B. Bloesch, W. Siegfried, A. Naef, M. Huber

Abstract: Disease management has been greatly improved with the new technologies of the
Internet. Current information and forecasting systems have not only a positive ecological, but an
economical one for wine growers as well. Now, it is possible to access the “VitiMeteo”
forecasting system free twice a day on the Internet ( The first “VitiMeteo”
module created was “VitiMeteo Plasmopara”. The core of this system is found in the database
“Agrometeo”, where all the data from different weather stations are stored. The purpose of
“Agrometeo” is to integrate weather data into various other software models. The next
component is “VitiMeteo Growth” which was programmed in cooperation with Hans-Reiner
Schultz from the Geisenheim Research Center. More software followed, such as “VitiMeteo
Insects”,”VitiMeteo Oidium” and “VitiMeteo Data Graph”. “VitiMeteo Insects” is a programme
that simulates the development of insects and other pests. Walter Kast, from the State Institute for
Viticulture, Oenology and Fruit Technology Weinsberg, was able to create an algorithm based on
“OiDiag-2.2”, which calculates which dates the spraying against powdery mildew (Erysiphe
necator) should begin and the time increments between each spray. Weather data are controlled
and presented with “VitiMeteo Data Graph”. The VitiMeteo-system is a cooperative project
between the State Institute of Viticulture and Enology, Freiburg (Germany), the Swiss Research
Station ACW, Changins-Wädenswil (Switzerland), and the company GEOsens, Ebringen
(Germany). The company Meteoblue (Basel, Switzerland) has provided weather forecasts, which
have been integrated with our software modules since 2009. This is the first time that an actual
forecast of biological processes e.g. incubation period or growth was possible. The requirements
of the “VitiMeteo” forecasting system can be entered quickly, making it an extremely flexible


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The expert system OiDiag-2.2 – a useful tool for the precise scheduling
of sprays against powdery mildew of vine (Erysiphe necator Schwein.)

W. K. Kast and K. Bleyer

Abstract: OiDiag-2.2 is a system of two tools. The first one helps the vine growers to find the
date for the start of the sprays against powdery mildew (Erysiphe necator). The second tool
calculates an index value considering the climatical and ontogenic inputs for the risk of powdery
mildew attack. Vine growers should get a table of relevant fungicides with values for the
maximal time lag after the spray, which is completely covered by these fungicides considering
these index values.


0.00 € an interactive platform for a better management
of grapevine diseases and pests

O. Viret, P.-H. Dubuis, A.-L. Fabre, B. Bloesch, W. Siegfried, A. Naef, M. Hubert, G. Bleyer, H.-H. Kassemeyer, M. Breuer, R. Krause

Abstract: To control the main fungal diseases in accordance with the epidemiological
development of the fungi in viticulture, the use of forecasting systems is one of the major
progresses. Agrometeo is an interactive platform ( and a general tool for
agriculture, including actual and historical weather data, modules for field crops, grapevine and
fruit orchards. The grapevine module contains forecasting for downy mildew and grape berry
moths; leaf area adapted spraying, growth development model, pesticides index, and descriptions
of the main diseases and pests. The forecasting modules use data from a weather station network
covering the whole viticulture area of Switzerland. Grapevine downy mildew forecasting is done
with Vitimeteo, a new expert-system for the forecast of grapevine downy mildew, designed by
the research institute of Freiburg (Germany), Agroscope ACW and the company GEOsens. The
software generates graphics and tables freely available for the growers on the Internet. Leaf area
adapted spraying was developed in analogy to the tree row volume concept on fruit orchards. The
experiments show a reduction of about 20 to 30% of the use of pesticides by calculating the
precise dose needed for a given leaf area. The module in allows a ready to
use solution for the calculation of the amount of plant protection products based on the width and
height of the leaf canopy and the row distance, depending on the registered amount indicated on
the package. The number of Agrometeo users is in expansion confirming the grower's interest.


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Modelling and experimenting crop protection decision workflows:
some lessons from GrapeMilDeWS research

O. Naud, P. Cartolaro, L. Delière, B. Léger

Abstract: The political roadmap about pesticides in France is to reduce quantities by 2, “if
possible”, before 2018. Research is needed to design and evaluate new pest management
solutions. A decision workflow system, name GrapemilDeWS was designed, at the plot scale, to
handle grapevine powdery and downy mildews. GrapeMilDeWS stipulates throughout the season
if and when fungicide sprayings should take place. GrapeMilDeWS has been experimented on a
network of plots in different French wine regions. We give some results about the number of
sprayings generated by GrapeMilDeWS and the crop protection performance obtained. We
discuss methodological aspects such as partnership and data required to test and check such
decision system.


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Impact des données météo de précision sur l’évaluation locale
du risque épidemique modelisé sur le vignoble.
Premiers résultats de validation spatiale des orages de grêle de mai 2009
sur les vignobles de Bordeaux et Cognac

M. Raynal, C. Debord, S. Guittard, M. Vergnes, J. Congnard, D. Grimal

Résumé: Les orages de grêle très marqués de ce printemps 2009, nous ont permis de tester sur le
terrain, les outils de validation spatiale des données météo pluviométrique à maille fine (1x1km).
Ces premiers tests, effectués sur la grêle à défaut de pouvoir les cibler sur des foyers de
développement maladie, révèlent la pertinence de ce travail d’enquête dans la mesure où ils
peuvent porter sur des phénomènes de type accidentel, ou fortement localisés.
La plate forme de saisie Web, incitant les techniciens et viticulteurs, à partager leurs
observations nous a également donné satisfaction car elle a fait l’objet d’un accueil favorable
auprès de la profession.
Du point de vue du zonage de la grêle, on retient que l’information radar permet de définir
le caractère grêlés ou indemne d’une parcelle avec un taux réussite proche de 75%. Le radar ne
permet cependant pas de cerner l’approche quantitative des dommages subis par la vigne. Ce
travail d’expérimentation participative en réseau sera prochainement appliqué aux thématiques
épidémiologiques liées à la prévision des risques d’épidémie.


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Long-term survival of Plasmopara viticola oospores
T. Caffi, V. Rossi, M. Lusitani

Abstract: A research was carried out in order to verify the long-term viability of Plasmopara
viticola oospores. It is commonly assumed that oospores may survive across grapevines growing
seasons, but no experimental evidence of this assumption has been provided to date. Grapevine
leaves were collected from untreated plants in Emilia-Romagna in fall 2004 to 2008. Fragments
of these leaves were kept in soil under natural conditions. In spring 2005 to 2009, part of the leaf
fragments collected in the previous autumn were used to evaluate oospore germination using the
floating disk bioassay; in spring 2009, the bioassay was also performed using the leaf residue
collected in fall 2005 to 2007. Results demonstrated that P. viticola oospores remained viable for
the whole period considered in this work, i.e., about 65 months (from fall 2004 to spring 2009).


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Dispersal of the sexual stage of Erysiphe necator in northern Italy
V. Rossi, T. Caffi, S. E. Legler, R. Bugiani, P. Frisullo

Abstract: Either by themselves or in combination with mycelium in the dormant buds, ascospores
produced in chasmothecia (the sexual fruiting bodies) of Erysiphe necator are an important source
of primary inoculum for grapevine powdery mildew disease. In northern Italy, E. necator
overwinters mainly as the sexual stage (i.e., mycelia are not usually present in dormant buds), but
no data are available on the abundance of chasmothecia in the vineyards. Therefore, the dispersal of
chasmothecia was studied in commercial vineyards of northern Italy, from 2005 to 2007 (16
combinations of 10 vineyards x 3 years); the vines were not sprayed with fungicide during this
study. The dispersed chasmothecia were collected on filter papers in funnels that were placed on the
trunk of affected grapevines from mid-August to the end of leaf fall. Each filter paper was
examined microscopically, and the chasmothecia were counted. The first chasmothecia of the
season were dispersed between late August and early October; cumulative numbers of the
chasmothecia dispersed subsequently increased (with different dynamics depending on the vineyard
and year) but dispersal stopped at the end of leaf fall. Over all three years and 16 vineyards,
chasmothecia averaged 3/cm2 of trap surface, with a maximum of 15/cm2. The numbers of mature
chasmothecia that dispersed roughly depended on the powdery mildew severity on leaves: high
numbers of chasmothecia were associated with disease severity ≥ 80%. According to estimates
based on the current data, vine bark in vineyards trained with a Guyot, Geneva double curtain, or
spurred cordon pruning system could contain till 18, 19, or 44 million chasmothecia/ha,


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Modelling the effect of the grapevine growth and susceptibility
on the dynamics of a powdery mildew epidemic

A. Calonnec, S. Schnee, P. Cartolaro, M. Langlais

Abstract: Simulations are performed to explore the sensitivity of epidemics of powdery mildew
of grapevine to variation of parameters related to the pathogen, the plant growth or the crop
management. For early inoculation, the three parameters linked to the sporulation (t), the
dispersion process (cid) and the plant vigour (vig) are the most discriminating. The parameter of
ontogenic resistance (τ) is less discriminating, and the height of shoot topping and the distance
between buds are not discriminating. We also examined the relationship between vine vigour and
disease variables at key periods in the epidemic process. An increase of the parameter of vigour
from 0.2 to 1 amounted to a higher number of leaves at flowering (Nflo) and a higher rate of
leaves emergence (RLE). The RLE was correlated with the rate of diseased leaves emergence and
with the severity of the disease at shoot topping, whereas Nflo was correlated to the diseased
leaves area at day 240. The percentage of young leaves during first sporulation event (s1s2) was
correlated to the rate of shoot development (RDS). These two variables from host allowed to
discriminate the years. The duration and dynamic of infectious tissue can considerably vary
function on the development of secondary shoots therefore on the climatic conditions and vigour.


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Consideration of dynamical plant-pathogen interactions for an
improved management of powdery mildew epidemics in grapevine

S. Schnee, J. Jolivet, A. Calonnec

Abstract: The current control of grape powdery mildew requires news strategies, able to limit the
pathogen development and requesting a reduced number of chemical sprayings. This study
proposes to exploit host plasticity in order to investigate the effect of a plant growth changing on
either the epidemic process, or organs susceptibility. An experimental plot combining two
cultivars, three rootstocks and two opposite crop management for creating a vigour differential
was surveyed regularly during the vegetative growth and different variables were measured to
characterise host growth and pathogen. Shoots coming from vinestocks localized in the different
vigour area were sampled for organ susceptibility assessment. Two groups of vigour were
obtained, not based on the established treatment but on the individual growth potential, that differ
mainly by the number of secondary leaves. The vigorous group presents the higher level of
disease, increasing with the appearance of secondary leaves. A low vigour modifies the
susceptibility of the tissues, by an earlier appearance of ontogenic resistance phenomenon. The
relevance of vigour control and the concomitant physiologist mechanisms are discussed as
integrative strategy in the grape powdery mildew management.


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Blackrot - downy mildew control in small vineyards in southern Switzerland
C. Gessler, M. Jermini

Abstract: Blackrot caused by Guignardia bidwelli is causing damages in recuperated vineyards
in southern Switzerland planted with the cultivar Isabella. The source of inoculum is attributed to
abandoned vineyards. Heavy and prolonged rains may also favor infection by Plasmopara
viticola on Isabella especially if a heavy load of primary inoculum can be expected. A 6 year
experiment was made to test and confirm minimal necessary fungicide applications and timing to
control Blackrot and downy mildew. Infection conditions during the period between flowering
and up to six week after, have led to heavy losses reaching 100% without adequate control. A
combination of the two pathogens was registered, with P. viticola bunch symptoms without
visible sporulation. We recommend therefore using fungicides or fungicide combinations with an
effect on both pathogens. Mostly two well timed sprays with an appropriate fungicide active
against both pathogens are sufficient to control fully the diseases on bunches and avoid loss.
Under particular rainy situation a third applications gave 100% control. Timing should be before
rain events leading to prolonged leaf wetness during the time period between flowering and six
week afterwards. Winegrowers in the area are not professional winegrowers or farmers, having
little specific knowledge, therefore they will apply fungicides only at particular date (Saturday)
following the instruction of the manufactures. Applications are made by knapsack sprayers and
vineyards are usually terraced irregularly often as pergola. Therefore we developed an easy
applicable scheme to determine application timing and an instruction system to determine
fungicide concentration so to avoid over and under dosaging. Here we present the most relevant
points we teach to our winegrowers.


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First study on the population genetic structure of Guignardia bidwellii
M. Jermini, A. Angst, M. Raynal, C. Gessler, G. Broggini

Abstract: The ascomycete Guignardia bidwellii is the casual agent of the black rot of grape.
Native to North America introduced to Europe in the early 19th century and today regularly
observed in France, Italy, Germany and southern Switzerland. The black rot is not generally
considered as an important disease but it is able to induce heavy crop losses particularly in
regions characterised by weather conditions favourable to Plasmopara viticola. Present studies
focused on the genetic structure of the pathogen population, to get a better understanding of the
epidemiology and life cycle of the disease. We successfully extracted and amplified DNA from
mummified berries and developed six SSR markers for genotyping of G. bidwellii isolates. The
SSR markers were used to study the pathogen populations of two Merlot vineyards in France and
a mixed variety vineyard (Merlot, Regent, Isabella, Solaris, Bianca and Chambourcin) in
southern Switzerland. All three populations were related and shared common SSR alleles. Gene
diversity was similar for the two French populations, and the Swiss population was nearly clonal
in a genotype frequently observed in France. Only a few private genotypes were found, therefore
the populations differed mainly in genotype frequency. Based on our data we challenge the
importance of sexual reproduction for the propagation of the fungus. The low genotype and allele
diversity observed could be explained either by a generally low genotype polymorphism in
Europe or by a low SSR marker polymorphism. More research is therefore needed to assess SSR
marker polymorphism on an American population, to develop additional SSR markers for a
higher genotype resolution and to understand an eventually difference in the genotype frequency
between leaf and berry infections.


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Methods for screening new Ampelomyces strains to be used
as biocontrol agents against grapevine powdery mildew

S. E. Legler, T. Caffi, L. Kiss, A. Pintye, V. Rossi

Abstract: Grapevine powdery mildew is a key disease all across Europe and is currently controlled
almost exclusively using fungicides. To develop a new biofungicide product against this disease,
caused by Erysiphe necator, a large culture collection of Ampelomyces strains collected worldwide
from different powdery mildew species was screened to assess for the first time the level of
variability of different characteristics of these biocontrol agents known to occur as natural
mycoparasites of powdery mildews in the field. This preliminary screening revealed a considerable
diversity in many characteristics of different Ampelomyces strains including both culture patterns
and mycoparasitic activities against the asexual and sexual stages of E. necator. The screening
protocol developed in this work could be used in further studies on Ampelomyces in order to
develop new effective commercial biofungicide products against powdery mildew infections of
grapevine and other crops.


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Water activity at the fruit surface: a potential indicator of
grape berry susceptibility to Botrytis cinerea

M. Fermaud, C. Deytieux-Belleau, J. Roudet, G. Darrieutort, L. Geny

Abstract: Water activity (Aw) is a physical property of a humid solid, which is of prime
importance in food microbiology. This parameter is an assessment of available water, unbound to
nutrient molecules, which allows damaging bacteria, yeasts and fungi to grow in a food product.
As most moulds which do not grow below an Aw level of ca. 0.8, Botrytis cinerea was unable to
grow on artificial media at an Aw of 0.93 and below. In 2008, in an experimental vineyard (Vitis
vinifera L. cv. Merlot noir) near Bordeaux, Botrytis bunch rot development was assessed and the
evolution of water activity was investigated at the surface of healthy grape berries during fruit
development. Our results showed that Aw decreased steadily from the berry herbaceous stage to
maturity and was correlated negatively and significantly (P = 0.01) with disease incidence.
Furthermore, an experiment under controlled conditions showed the significant effect of relative
humidity (RH) on the water activity level at the berry surface. When healthy berries at two different
development stages (“herbaceous” and “beginning of colour change”) remained at 80 % RH for 6
days, the Aw values decreased significantly (P = 0.05). On the basis of the close relationship
between water activity and Botrytis bunch rot development, the potential of Aw for use as a new
disease indicator is discussed as well as the influence of climatic conditions on water activity
measured at the grape berry surface.


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Investigation of contamination pathways of Esca-associated fungal pathogens
and of fungicide-based control strategies

A. Kortekamp, J. Köckerling

Abstract: Even though “Esca” may be as old as viticulture itself, new attention has been directed
to this disease in recent years due to the dramatic increase in economic importance. A sudden
wilting of Esca-affected grapevines, followed by the death of the entire plant, occurs in many
grapevine-growing areas of the world. Esca is a complex disease that comprises several
symptoms caused by a set of fungal pathogens. Unfortunately, infected plants can often not be
cured, since foliar or wood treatments using fungicides do not lead to an effective control of the
respective pathogens in the infested wood. Given the large number of wounds made to
propagation material during the various nursery stages and also made as a result of repeated
pruning, hygiene and wound protection is of particular importance. Therefore, the effect of
various chemical and biological treatments on Esca-associated fungi, such as Phaeoacremonium
aleophilum (Pal), Phaeomoniella chlamydospora (Pch), and Fomitiporia mediterranea (Fmed),
and on the disease progress of artificially infected plants was investigated in vitro and in field and
greenhouse experiments. For this purpose, several putative contamination pathways and different
test systems have been evaluated to verify the ports of entry for fungal spores and the efficiency
of fungicides to control the disease.
In the year 2009, vineyards of the DLR research station and of local grape growers were
inspected and the impact of Esca in these vineyards was evaluated. As published by other
authors, the number of affected vines increased with their age. Furthermore, there are no clear
differences in susceptibility and none of the varieties planted in the Palatinate showed any
suitable resistance. Plant material of diseased vines was collected and tested for the presence of
Esca-inducing pathogens. Mainly Phaeomoniella chlamydospora (Pch), Botryosphaeria species,
Cylindrocarpon sp., Trichoderma sp. and mould fungi such as Alternaria sp. and Penicillium sp.
were frequently found. All type of wounds created artificially represented suitable ports of entry
for all Esca-associated pathogens. Especially, infections with Cylindrocarpon destructans
significantly reduced viability of the scions and had severe effects on plant growth as tested with
infected cuttings. Wound infections caused by polluted water that had been used to soak
propagation material led to a reduced growth of cions, especially when contaminated with
Cylindrocarpon spores.
Growth tests in vitro showed that most fungicides tested exhibited antifungal capacities. The
Trichoderma-based product Trichostar® was an effective biological agent in the field, preventing
infection of wounds when applied one day before inoculation, whereas most fungicides were not
able to sufficiently prevent colonisation of wounds by fungi.


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Impact of biotic and abiotic factors on the development of Esca decline disease
P. Lecomte, G. Darrieutort, C. Laveau, D. Blancard, G. Louvet, J.-P. Goutouly, P. Rey, L. Guérin-Dubrana

Abstract: The aim of researches performed by our group is to determine which internal and
external factors have a key influence on grapevine wood diseases, especially on Esca. The
relationship between Esca leaf symptoms, the size of inner necrosis, the fungal endophytes
associated with the disease and the whole microflora that can be detected from the grapevine
wood, is discussed. Recent studies on leaf symptom development showed that summer
temperature conditions likely play a major role in the expression of Esca symptoms. Cultural
factors, like plant vigor or type of soils, also have presumably a significant influence. Several
evidences suggest that other abiotic factors linked to the training systems (e.g. forms with very
short cordons) or to pruning decisions (e.g. winter hand pruning characteristic of the modern
grape-growing training systems) have also to be taken into account, alone or combined with other
factors. Whole data indicate that Esca is a multi-factor disease and that many complex
pathological scenarios could explain the grapevine trunk disease expression. A control strategy,
mainly based on cultural measures to avoid a too early development of inner necrosis, is


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Electrospun nanofibers as novel carriers of insect pheromones:
communication disruption strategy against Lobesia botrana

D. F. Hein, M. Breuer, H. E. Hummel, A. Greiner, J. H. Wendorff, C. Hellmann, A. Vilcinskas, A. Kratt, H. Kleeberg, G. Leithold

Abstract: Using organic nanofibers as dispensers for pheromones and kairomones in plant
protection for disrupting insect chemical communication is a novel approach aiming at popularizing
this technique in organic and integrated plant production. Expected advantages of the nanofibers are
highly controlled spatiotemporal release rates of pheromones / kairomones, improved climatic
stability, and mechanized application. Dispenser types used so far show deficiencies in one or more
of these requirements. Mechanical application of pheromone dispensers is a new approach to
reduce the costs of manual labour. Therefore the environmentally compatible, highly specific and
efficient technique of mating disruption may become an alternative to the use of synthetic pesticides
in integrated pest management. The electrospun nanofibers are highly elastic, which prevents
break-off of smaller pieces, and polymers used are biocompatible. Due to the tiny scale of
nanofibers the mass input both for pheromones and for polymeric nanofibers is extremely low.
Major environmental benefits are high control specificity, very low concentrations of residues and
minimal risk towards development of resistant insect strains. We tested organic polymer fibers,
made from the biodegradable polymer Ecoflex® (BASF), treated with Lobesia botrana
(Lepidoptera: Tortricidae) sex pheromone, (E,Z)-7,9-dodecadienyl acetate, in cage tests placed
inside vineyards. With this experimental setup it is possible to show the applicability of mating
disruption dispensers for the use in plant protection against insect pests in vineyards. We used
Isonet LE dispensers (Shin-Etsu) as a positive control. The Ecoflex® nanofiber-pheromonedispensers
show a mating disruption effect which is comparable to the efficacy of the Isonet LE
dispensers for at least three weeks. After that, the mating disruption effect observed tapers off to a
level where it is insufficient for plant protection purposes. But, in principle, our first prototype of a
nanofiber pheromone dispenser has proved its efficacy for mating disruption purposes.
Modifications of the fibers, currently under development, open up the possibility of extending the
disruption effect to a period of several months’ duration, enough to cover the entire flight period of


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Control of grape berry moth larvae using parasitoids: should it be developed?
D. Thiéry, L. Delbac, C. Villemant, J. Moreau

Abstract: Besides mating disruption techniques and progress in monitoring techniques (e.g. the
use of food traps against females), biological control may reveal itself very efficient at controlling
grape moth populations. Parasitoids active to control grape moths are known for long in
vineyards; few of them were already described in the middle of the 19th century in French
vineyards and their efficiency was already recognized especially against the diapausing and the
first spring generations of the moths. Rather numerous attempts to release egg parasitoids have
been done in different European countries using different species of trichogrammas. The results
obtained varied a lot and could not yet clearly promote the use of this technique in vineyards. We
believe that a biological control based on larval parasitoids could efficiently be developed as a
valuable alternative to chemical control. In the present paper, we focus on larval parasitoids
among which ichneumonids and chalcidoids (Hymenoptera) dominate, and present results
obtained in different French vineyards (Bordeaux vineyard, Perpignan and Montpellier area,
Côtes du Rhône and Alsace). We discuss factors that may favour or reduce their efficiency as
biocontrol agents.


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Life history of Lobesia botrana on Daphne gnidium in a Natural Park of Tuscany
A. Lucchi, L. Santini

Abstract: The life cycle and the ecology of the grapevine moth (GM) Lobesia botrana (Den. &
Schiff) feeding on Daphne gnidium L. (to date considered the original host for L. botrana) were
investigated in a Tuscan Natural Park in the last 2 years. The studied environment was located 2
km away from the beach and hosted numerous shrubs of D. gnidium on which L. botrana has
been feeding forever, in the absence of Vitis vinifera. In the mentioned environment the moth
seasonal flights were monitored and the larval feeding behaviour along the whole season was


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Current status of grapevine leafminers in north-eastern Italy
C. Duso, A. Pozzebon, M. Baldessari, G. Angeli

Abstract: Up to 2007, two species of grapevine leafminers were known in Italy as well as in
Europe: Phyllocnistis vitegenella Clemens and Holocacista rivillei (Stainton). They are usually
considered minor pests and do not require specific control measures. However, an increasing
importance of these pests has been observed in north-eastern Italy in recent growing seasons. An
additional leafminer species belonging to the genus Antispila was also detected in 2007 in northeastern
Italy. The origin of this species, which is new for Europe, is still unknown. The phenology,
pest status, and natural control of these leafminer species have been investigated in a number of
vineyards located in the Veneto and Trentino regions. The implications of leafminer occurrence for
integrated pest management (IPM) on grapevines are discussed.


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Factors affecting the post-release dispersal of Trichogramma cacoeciae Marchal
in the vineyard

G. Hommay, J. C. Kienlen, C. Gertz, C. Bihry, J. Pizzol

Abstract: Several releases of 20,000 and 40,000 individuals of Trichogramma cacoeciae were
made at a central point in a vineyard in order to follow the dispersal of trichogrammes and the
distribution of parasitism on Ephestia kuehniella egg-cards. The dispersal of trichogrammes
essentially occurred along the release row and over the nearest rows, since the vine rows formed a
plant screen that channelled their dispersion. The daily captures of trichogrammes increased with
the number of trichogrammes released and decreased over time. It increased with minimal
temperature and solar radiation, but did not depend on other meteorological factors. The number of
parasitised egg-cards decreased with the distance from the release point and increased with the
amount of wind received at the control points. The doubling of the number of trichogrammes
released influenced parasitism by increasing both the discovery rate and the exploitation rate of


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Mating disruption field trials to control the vine mealybug Planococcus ficus
A. Cocco, M. Coinu, A. Lentini, G. Serra, G. Delrio

Abstract: A mating disruption experiment against the vine mealybug, Planococcus ficus, was
carried out during 2008 in 3 commercial vineyards in northwestern Sardinia. Within each
vineyard, 2 adjacent plots of variable size (0.2, 0.4, and 0.5ha, respectively) were established as
mating disruption and control plot. Plastic dispensers (Suterra Inc., USA) with 100mg each of the
synthetic sex pheromone were deployed in mid-May, before the first flight of adult males, at a
rate of 625 dispensers per hectare. The effectiveness of the mating disruption technique was
evaluated by comparing the number of males captured in pheromone traps, the leaf population
density, the percentage of ovipositing females, and the crop damage. Male flights were monitored
with traps baited with 0.01mg of the sex pheromone. The percentage of females with ovisacs and
their fecundity were estimated by collecting adult females after each peak male flight and rearing
them individually under laboratory conditions. The mealybug density was assessed from June to
September by counting every 2 weeks nymph and female mealybugs on basal leaves of 20-30
vines per plot. The crop damage was evaluated at harvest by assessing the percentage of damaged
bunches on the same vines. In all the vineyards, the number of males captured in mating
disruption plots was lower by far than in control plots, while the percentage of females with
ovisac was significantly different only in the first generation. Females collected from the field
after the first male flight produced more eggs than those collected after the second and third
flight, with no significant difference between treatments. The density of mealybugs on leaves in
mating disruption plots was lower than that in control plots, but the difference was not
significant. Also, the percentage of damaged bunches at harvest did not differ between
treatments. Mating disruption experiments to control the vine mealybug produced mixed results.
This could be due to the limited size of experimental plots and/or the suspected parthenogenetic
reproduction of Sardinian populations of P. ficus.


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Biological characteristics of Heliococcus bohemicus and
Ericydnus sipylus in controlled conditions

A. Fleisch, L. Pérez, P. Kreiter, A. V. Odor, G. Sentenac

Abstract: Heliococcus bohemicus is an increasing pest in vineyard in the north-east of France. A
biological control was initiated in 2007 and 2008. A study in laboratory, to measure the two
antagonist biological characteristics, the mealybug and one of its parasitoids Ericydnus sipylus,
was undertaken. The description of the mealybug life cycle is led under the climatic conditions:
25°C and 60% R. H. It appears that H. bohemicus has three larvae instars. Females did not lay off
eggs and the larvae hatched after 23.5 days on average. The development of first and second
instars lasted near 34 days and for the third instars it was near 14 days. The biological
characteristics of the parasitoid were studied at 21°C and 50% R. H. Under these conditions the
pre-imaginal development lasted 35 days and the length of adult emergence was 57 days on
average. The fertility is equal to 15 individuals. Others parameters are studied and are explained
in the article. The method of the mass rearing is developed. The pest insect is developed on
potato tubers turned green by light.


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Survey on Scaphoideus titanus egg distribution on grapevine
B. Bagnoli, E. Gargani

Abstract: In the context of the Euphresco-Propscaph project, a survey on Scaphoideus titanus
egg distribution on grapevine has been under way since January 2009. Samples of one and two to
four-year old vine wood of the “Trebbiano Toscano” variety from an untreated vineyard in
Latium (Central Italy) and samples of one and two-year old of the “Kober 5BB” rootstock from
an abandoned field in Veneto (North Italy) have been analysed. The vine samples, collected
during the winter pruning, were stocked at 4°C till the beginning of the experimentation, when
the same materials were cut into pieces of different lengths and put into rearing boxes (cm
29x20x12) maintained at 24°C, 75% RH and LD 16:8 photoperiod to obtain the first instar
S. titanus larvae. In the middle of May in two Latium untreated vineyards, several original
capture devices were put on the plants, in particular around the trunk, the cordon, the cane of oneyear
old and the buds of the vines to survey the emergence of S. titanus larvae. Results confirmed
that S. titanus females prefer to oviposit in the bark of two or more year old wood (0.09-0.31
larvae per cm2), even if very few specimens were obtained from the one-year old wood (0.0002-
0.0036 larvae per cm2) as well. The field experiments with the capture devices evidenced for the
first time that even the bark of the trunk is a preferential site of the cicadellid oviposition as well.


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Preliminary study of the aggregative behaviour of Scaphoideus titanus larvae
J. Chuche, A. Boursault, D. Thiéry

Abstract: The leafhopper Scaphoideus titanus is the vector of the Flavescence dorée. In this study,
we investigated the aggregative behaviour of the larvae. We conclude from different experiments
that larval aggregation occurs at the plant scale and the age and colour of the food source could be
factors cueing an aggregation. These aggregation patterns should be studied in more details in order
to gain knowledge in the epidemiology of Flavescence dorée and eventually to develop control
strategies based on inter-individual epideictic regulation.


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Vibrational signals associated to the mating behaviour
of Hyalesthes obsoletus Signoret (Hemiptera Fulgoromorpha)

V. Mazzoni, G. Anfora, C. Ioriatti, M. Virant-Doberlet, A. Lucchi

Abstract: Hyalesthes obsoletus Signoret is a vector of the stolbur phytoplasma (group 16SrXIIA),
causal agent of the grapevine disease ‘Bois Noir’. In planthoppers the intraspecific
communication is based on substrate-borne vibrational signals. The aim of this study was to
investigate the mating behaviour of H. obsoletus. Vibrational signals were detected from nettle
cuttings by laser vibrometer. Both male and female can start the mating process by the emission
of calling signals. The male calling signal is a single short syllable (Male Syllable 1) whereas the
female’s is a train of pulses of variable length. Initially, the male/female duet is based on the
alternating emission of calling signals (recognition phase). In a second step, the male starts
emitting long syllable trains (male courtship signal) in which the female occasionally inserts
single pulses (courtship phase). The male courtship signal is made of two different types of
syllables (Male Syllable 2 and 3), the switch from one to another often triggered by the emission
of a female pulse. When partners are in close vicinity the male emits a specific pre-copula signal
that precedes the copula (pre-copula phase). Rivalry tests, either pairs of males or trios, showed a
strong competition between males, expressed in the form of chorusing, rivalry signals and


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A beneficial species becomes a pest - the common earwig
Forficula auricularia (Linnaeus 1758)

C. Huth, K.-J. Schirra, A. Seitz, F. Louis

Abstract: The common earwig Forficula auricularia has been classified as a beneficial predator
in vineyards. Amongst others the insect feeds on grape pests like different tortricids. In recent
years within many regions of the viticultural area of the Palatinate the individual densities
increased to an extremely high level. Earwigs may cause direct damages such as contamination
of the grapes with faeces, eroded berries and transfer of pathogens. The chemical agent 2-methyl-
1,4-benzoquinone, released from the abdominal glands while earwigs are menaced and likewise
contained in faeces, may have a negative influence on the wine quality. All these facts constitute
a deterioration of grape quality for wine-growers. The high number of earwigs in the grapes after
vintage causes a negative image by consumers. This study was carried out to investigate possible
relations between the population dynamics of earwigs and specific environmental conditions in
vineyards. The main focus of the research project was focussed on chemical, ecological and
biological strategies to reduce the population densities. Another important point of survey was to
study the life cycle of earwigs especially in vineyards. For sampling purposes in the cluster zone
a special life trap made of bamboo tubes has been developed. This type of trap showed the
highest catch rate of the four trapping types tested. For monitoring the ground dwelling earwigs
pitfall traps were used.


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Effects of some insecticides on Kampimodromus aberrans:
laboratory and field studies

P. Tirello, S. Vettore, A. Pozzebon, M. Lorenzon, C. Duso

Abstract: Kampimodromus aberrans is an important predatory mite in European vineyards and
fruit orchards. Pesticides can exert profound effects on K. aberrans populations that are
influenced by the pesticide experience of predatory mites. Resistance to organophosphates and
dithiocarbamates has been suggested for some Italian populations of K. aberrans but this
phenomenon has not been shown in the laboratory. The effects of three different pesticides
(chlorpyriphos-ethyl, methoxyfenozide and flufenoxuron) on K. aberrans were investigated in
field and laboratory conditions. In field tests, predatory mites were released at the beginning of
February on defined plots. Kampimodromus aberrans densities were evaluated on insecticide
treated plots and on control plots by examining leaf samples in the laboratory from June to
August. The lethal and sub-lethal effects of the same pesticides were tested in the laboratory
using a residual bioassay. Field experiments showed some effects of the three pesticides on
predatory mite populations. Results of bioassays were partially correlated with those obtained in
the field. Implications for IPM are discussed.


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Development and reproduction of the predatory mites
Kampimodromus aberrans, Typhlodromus pyri and Amblyseius andersoni
on different food sources

M. Lorenzon, A. Pozzebon, C. Duso

Abstract: Kampimodromus aberrans, Typhlodromus pyri and Amblyseius andersoni, important
predatory mites in European vineyards, were reared in the laboratory on five potential food
sources: the herbivores Panonychus ulmi and Eotetranychus carpini, the non-specialized feeder
Tydeus caudatus, the pollen of Typha latifolia, and the mycelium of grape downy mildew
Plasmopara viticola. Experiments were conducted using grape leaves as a substrate. The
developmental times, survival and fecundity of predatory mites were determined on each food
type. None of these species developed on T. caudatus. Developmental times of K. aberrans and
T. pyri were faster on pollen than on spider mites or P. viticola mycelium. The fecundity of
K. aberrans and T. pyri was higher on pollen than on other foods. Amblyseius andersoni
developed faster on pollen than on P. ulmi or P. viticola mycelium, and showed a low fecundity
on P. viticola mycelium.


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Susceptibility and sensibility of grape cultivars to the leafhopper Empoasca vitis
D. Fornasiero, A. Pozzebon, F. Pavan, C. Duso

Abstract: The leafhopper Empoasca vitis (Goethe) (Homoptera: Cicadellidae) is a serious pest in
European vineyards. The assessment of economic damage caused by E. vitis was associated with
no definite results probably because of a different susceptibility and sensibility of grape cultivars
to the pest. Empoasca vitis abundance and symptoms associated with the activity of this pest
were evaluated during three years on 10 grape cultivars grown in collections located in northeastern
Italy. Empoasca vitis densities were estimated from May-June to September by sampling
leaves every 7-10 days. At vintage time, the percentage of leaf surface with symptoms caused by
E. vitis feeding was estimated. Carménère, Sauvignon Blanc and Tocai Friulano showed to be
very susceptible and very sensible, whereas Cabernet Sauvignon, Chardonnay, Verduzzo Friulano
and Pinot Gris showed an opposite behaviour. However, such correspondence was influenced by
meteorological conditions and plant stress factors. These results may be useful for establishing
economic thresholds and sampling plans for E. vitis in vineyards.


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Can Harmonia axyridis affect the taste of European wines?
C. Linder, F. Lorenzini, P. Kehrli

Abstract: The multicoloured Asian ladybeetle Harmonia axyridis Pallas is now established in
Central Europe and is likely to spread soon over the whole continent. In the United States, H.
axyridis moves to vineyard shortly before harvest. When disturbed or crushed during vinification,
the ladybeetles release alkylmethoxypyrazines, which can strongly affect the quality of white and
red wines. In Europe, wine growers are beginning to worry about the impact of H. axyridis on the
quality of their processed wines. In this study we artificially contaminated harvested grapes of the
variety 'Chasselas' and 'Pinot noir'', the two main varieties of Switzerland, with three different
densities of H. axyridis and determined the impact of crushed ladybeetles on the taste of
processed wines. The addition of ladybeetles did neither affect fermentation nor the basic
chemical properties of processed wines. However, at the organoleptic level the contamination of
grapes had a significant effect. Contaminated 'Chasselas' wines were of lower fruitiness, fineness,
structure, acidity, equilibrium and exhibited a "rancid oil" odour. Moreover, the quality of
contaminated 'Pinot noir' was rated low, in particular, its fruitiness, spiciness, fineness, structure,
softness, robustness as well as intensity and quality of tannins was poor. Even though H. axyridis
is rarely present in vineyards of Western Switzerland, our results emphasise that there is a risk of
wine contamination. Thus, the evolution of the multicoloured Asian ladybeetle in European
vineyards should be carefully monitored in order to anticipate future problems.


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Feeding behaviour of Lobesia botrana on leaves and shoots of grapevine
A. Lucchi, E. Pozzolini, G. Anfora, V. Mazzoni, M. Tasin, E. Leonardelli, C. Ioriatti

Abstract: The larval feeding behaviour of the grapevine moth Lobesia botrana (GM) on
grapevine leaf and shoot tissues in the absence of grapes is described. GM larvae were obtained
from eggs laid on the grapevine leaves in a field cage covering two rows of a Chardonnay
vineyard in which all the bunches were previously removed. Larvae developed till pupation
feeding only on leaves and shoots and showed the characteristic leaf rolling habit of the
Tortricidae. All of them were able to pupate and to give adults that mated in laboratory
conditions. Females laid fertile eggs and gave rise to a new generation of larvae. Pupal weight,
number of eggs laid, and longevity of leaf/shoot fed insects were compared with those of the
insects reared on berries and artificial diet.


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Attractiveness of different colours to Scaphoideus titanus Ball
(Hemiptera: Cicadellidae) adults

V. Mazzoni, F. Trona, C. Ioriatti, A. Lucchi, A. Eriksson, G. Anfora

Abstract: Laboratory and field tests were performed to ascertain a possible role of visual cues
for Scaphoideus titanus during the location of a host plant. In laboratory, two choice colour tests
were made in a Y-tube to compare the attractiveness of blue (peak wavelength: 475nm), green
(521nm), yellow (573nm) and red (650nm). The observed preference order was yellow > red >
green > blue for males and red > yellow > green > blue for females. In field, nine groups of three
coloured (yellow, red, blue) sticky traps were positioned in an organic vineyard and replaced
once a week, for two summers (2008 and 2009). In both seasons the yellow traps captured
significantly more individuals (either males or females) and had the highest sex rate (percentage
of males on the total captures) followed by red and blue. Our investigation suggests that, despite
a partial different response between laboratory and field tests, S. titanus adults are influenced by
visual cues present in their habitat. The possible reasons of the different responses observed in
lab and field are discussed.


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The impact of a number of insecticides on Empoasca vitis populations
in north-eastern Italy

A. Pozzebon, M. Pederiva, R. Moret, C. Duso

Abstract: The green leafhopper Empoasca vitis Goethe (Homoptera: Cicadellidae) is an important
pest of grapes in Europe. Its feeding activity may cause a reduction in photosynthesis, mesophyll
conductance and transpiration rate with implication for yields. For these reasons insecticides are
widely used to control this pest with potential effects on beneficial arthropods. Field experiments
were conducted to evaluate the impact of a number of insecticides (chlorpyriphos, flufenoxuron,
thiamethoxam, and indoxacarb) on E. vitis populations. Pesticide side-effects on the predatory mite
Typhlodromus pyri were assessed. Thiamethoxam was the most effective towards E. vitis. Pest
population levels were slightly reduced by chlorpyriphos, suggesting that this strain was resistant to
organophosphates. Indoxacarb and flufenoxuron were associated to intermediate effects. The
predatory mite populations were affected especially by thiamethoxam.


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Small insect enclosure field cages: A simple method to assess mating disruption
F. Briand, C. Vergely, P.-J. Charmillot, P. Kehrli

Abstract: Mating disruption is an effective and sustainable integrated pest management technique
that is now established in a broad range of cropping systems. For example, it is a popular alternative
to insecticides for controlling the grape berry moth Eupoecilia ambiguella Hb. (Lepidoptera,
Tortricidae), a main pest of European vineyards. A downside of this environmental friendly pest
control technique is the challenging assessment of its efficiency in the field. In order to obtain
reliable results, newly processed pheromone dispensers have to be tested at a large spatial scale
where pest densities, crop variety, cultural practices, microclimate etc. can vary considerably
among trial sites. The realisation of statistically sound results therefore asks for numerous
independent replicates, which are time-consuming, space-demanding and expensive. The aim of
this work was to facilitate the evaluation of the efficiency of mating disruption. We tested the
suitability of small insect enclosure field cages for a pre-evaluation of the impact of sex
pheromones on insect mating. Our enclosure field cages consisted of a cubic metal frame of 35cm
side length that was covered with gauze tissue. Cages were installed in the centre of pheromone
treated and untreated vineyards. Thereafter, various numbers of grape berry moth couples were
exposed for 1, 2 or 3 days. The impact of pheromones was assessed by counting the number of
mated females, e.g. the number of dissected spermatophores. Our data showed that mating was
significant lower in pheromone treated compared to untreated vineyards. Moreover, there were
significant differences among pheromone dispensers tested and mating disruption generally
increased with the amount of pheromone diffused. Overall, the exposure of eight couples for one
night seemed to be optimal for comparing different control schemes. Our study showed that small
insect enclosure field cages offer a fast, simple, cheap and reliable method to measure the impact of
pheromones on insect mating. Data obtained in such trials may therefore help predicting the
value/use of setting-up large-scale field trials. In conclusion, small insect enclosure field cages may
facilitate the development and assessment of semiochemical lures.


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Influence of nettle control along a ditch on spatial distribution
of Hyalesthes obsoletus Signoret in a neighbouring vineyard

N. Mori, N. Reggiani, A. Pozzebon, C. Duso, F. Pavan

Abstract: The nettle (Urtica dioica L.) is the most important host plant for the phytoplasma
associated with the Bois noir (BN) disease of the grapevine and for its vector, Hyalesthes
obsoletus Signoret. A higher abundance of the vector inside the vineyards is favoured by the
nettle growing in edge rows and surrounding ditches. Foliar applications of insecticides appeared
not an effective measure for vector and disease control, whereas nettle control could be useful to
reduce BN incidence in vineyards. In particular the efficacy of nettle control by selective
herbicide applications in early autumn and early spring was demonstrated. Many authors have
suggested that the nettle control applied during H. obsoletus flight could have negative effects,
because the vector adults could be forced to migrate onto grapevines. The aim of this research
was to evaluate if nettle cutting during the adult flight favours the colonisation of the vineyards
by the vector. For this purpose, three different weed managements (no cut, one cut during adult
flight, frequent cuts) were applied on the herbaceous vegetation of a ditch bordering a vineyard.
In this ditch, nettle was one of the principal components. The flight dynamic of H. obsoletus was
recorded by yellow sticky traps placed on herbaceous vegetation along the ditch and at different
positions inside the vineyard. Results showed that nettle cutting induced a reduction in vector
presence in the ditch, but favoured its dispersion inside the vineyard. The implications of these
observations on disease control are discussed.


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Electropenetrography, a tool to investigate the feeding behaviour
of sucking insects: development of this technique to Scaphoideus titanus

J. Chuche, N. Sauvion, D. Thiéry

Abstract: The leafhopper Scaphoideus titanus is the vector of the Flavescence dorée and
transmits the phytoplasma causing the disease while feeding. To better understand the
transmission process, we developed for this leafhopper the electropenetrograpy technique which
allows studying the feeding behaviour. We used the direct current method (DC system) to study
the fundamental processes of the S. titanus feeding behaviour. We present the first results of the
characterization of the electrical patterns obtained with this insect.


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Gaps in knowledge for modern integrated protection in viticulture:
lessons from controlling grape berry moths

Denis Thiéry

Abstract: Viticulture has an impressive range of progress for the future years concerning the use
of integrated pest management. This can be illustrated by insect pest management for which
several tools have been developed or could be developed rather soon for an integrated protection
management. The present talk aims at presenting through the experience gained in on insect pest
or vectors management gaps and potential progress that can be achieved. The European
grapevine moth Lobesia botrana can serve as a case study to analyse the different possibilities
that can be used in IPM. Few existing examples of IPM compatible techniques will be presented:
use of behaviour modifying chemicals in push-pull strategies, monitoring techniques,
mathematical models, biotechnical insecticides (Bt) and natural enemies.
The different advantages and limits are discussed, and we attempt to identify gaps where
scientific or development efforts are required to progress.


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